human chondrocytes hc Search Results


96
Cell Applications Inc muscle cell basal medium cell applications
Muscle Cell Basal Medium Cell Applications, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Cell Applications Inc human chondrocytes hc
<t>Chondrocyte</t> differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.
Human Chondrocytes Hc, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Cell Applications Inc primary human oa articular chondrocytes
<t>Chondrocyte</t> differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.
Primary Human Oa Articular Chondrocytes, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
China Center for Type Culture Collection human chondrocytes hc
<t>Chondrocyte</t> differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.
Human Chondrocytes Hc, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
ScienCell human articular chondrocyte cell line hc-a
<t>Chondrocyte</t> differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.
Human Articular Chondrocyte Cell Line Hc A, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Avantor p4-p5 human chondrocyte (hc
<t>Chondrocyte</t> differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.
P4 P5 Human Chondrocyte (Hc, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
European Collection of Authenticated Cell Cultures stable cell line of adult human articular chondrocytes hc-402-05a
HiPSCs were differentiated through four different protocols: a monolayer culture with a set of defined growth factors (GF) (DIRECT protocol); b embryoid bodies (EB) with the exogenous addition of TGF-β3 (TGF-β3 protocol); c EBs in a medium conditioned on the HC-402-05 cell line (COND protocol); and d EBs in a conditioned medium supplemented with TGF-β3 (TGF-β3+ COND protocol). In all cases ( a , b , c , d ), the cells obtained through these differentiation processes presented morphological characteristics of <t>chondrocytes</t>
Stable Cell Line Of Adult Human Articular Chondrocytes Hc 402 05a, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Cambrex human chondrocyte (hc) cells
HiPSCs were differentiated through four different protocols: a monolayer culture with a set of defined growth factors (GF) (DIRECT protocol); b embryoid bodies (EB) with the exogenous addition of TGF-β3 (TGF-β3 protocol); c EBs in a medium conditioned on the HC-402-05 cell line (COND protocol); and d EBs in a conditioned medium supplemented with TGF-β3 (TGF-β3+ COND protocol). In all cases ( a , b , c , d ), the cells obtained through these differentiation processes presented morphological characteristics of <t>chondrocytes</t>
Human Chondrocyte (Hc) Cells, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
ScienCell hmsc and human chondrocytes (hc-a, cat. no. 6884)
HiPSCs were differentiated through four different protocols: a monolayer culture with a set of defined growth factors (GF) (DIRECT protocol); b embryoid bodies (EB) with the exogenous addition of TGF-β3 (TGF-β3 protocol); c EBs in a medium conditioned on the HC-402-05 cell line (COND protocol); and d EBs in a conditioned medium supplemented with TGF-β3 (TGF-β3+ COND protocol). In all cases ( a , b , c , d ), the cells obtained through these differentiation processes presented morphological characteristics of <t>chondrocytes</t>
Hmsc And Human Chondrocytes (Hc A, Cat. No. 6884), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chondrocyte differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.

Journal: Experimental and Therapeutic Medicine

Article Title: Ajuga decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model

doi: 10.3892/etm.2018.5981

Figure Lengend Snippet: Chondrocyte differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.

Article Snippet: Human chondrocytes (HC) derived from normal human femoral cartilage were obtained from Cell Applications, Inc. (Merck KGaA).

Techniques: Cell Culture, Staining

Effect of EADE on PGE2 production in chondrocytes. Human chondrocytes were stimulated with IL-1β (1 ng/ml) and treated with 10 or 100 µg/ml EADE. The concentration of PGE2 was significantly increased by IL-1β stimulation, and EADE significantly suppressed this increase. *P<0.05. IL, interleukin; EADE, extra Ajuga decumbens extract; PGE2, prostaglandin E2.

Journal: Experimental and Therapeutic Medicine

Article Title: Ajuga decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model

doi: 10.3892/etm.2018.5981

Figure Lengend Snippet: Effect of EADE on PGE2 production in chondrocytes. Human chondrocytes were stimulated with IL-1β (1 ng/ml) and treated with 10 or 100 µg/ml EADE. The concentration of PGE2 was significantly increased by IL-1β stimulation, and EADE significantly suppressed this increase. *P<0.05. IL, interleukin; EADE, extra Ajuga decumbens extract; PGE2, prostaglandin E2.

Article Snippet: Human chondrocytes (HC) derived from normal human femoral cartilage were obtained from Cell Applications, Inc. (Merck KGaA).

Techniques: Concentration Assay

HiPSCs were differentiated through four different protocols: a monolayer culture with a set of defined growth factors (GF) (DIRECT protocol); b embryoid bodies (EB) with the exogenous addition of TGF-β3 (TGF-β3 protocol); c EBs in a medium conditioned on the HC-402-05 cell line (COND protocol); and d EBs in a conditioned medium supplemented with TGF-β3 (TGF-β3+ COND protocol). In all cases ( a , b , c , d ), the cells obtained through these differentiation processes presented morphological characteristics of chondrocytes

Journal: Stem Cell Reviews

Article Title: Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)

doi: 10.1007/s12015-016-9708-y

Figure Lengend Snippet: HiPSCs were differentiated through four different protocols: a monolayer culture with a set of defined growth factors (GF) (DIRECT protocol); b embryoid bodies (EB) with the exogenous addition of TGF-β3 (TGF-β3 protocol); c EBs in a medium conditioned on the HC-402-05 cell line (COND protocol); and d EBs in a conditioned medium supplemented with TGF-β3 (TGF-β3+ COND protocol). In all cases ( a , b , c , d ), the cells obtained through these differentiation processes presented morphological characteristics of chondrocytes

Article Snippet: In all analyses, a stable cell line of adult human articular chondrocytes (HC-402-05a) served as a positive control based on the recommendations of the European Collection of Authenticated Cell Cultures (ECACC) for evaluation of the differentiation process in in vitro model systems ( https://www.pheculturecollections.org.uk/products/celllines/primarycells/detail.jsp?refId=06090702&collection=ecacc_nepc ).

Techniques:

The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND and TGF-β3 + COND were analyzed by qPCR. The cells did not express Nanog , OCT4 , SOX2, or E-cadherin a , the genes responsible for maintaining pluripotency. The differentiated cells expressed chondrogenic genes: type II collagen , SOX5 , SOX6 , SOX9 and NKX3.2 b

Journal: Stem Cell Reviews

Article Title: Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)

doi: 10.1007/s12015-016-9708-y

Figure Lengend Snippet: The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND and TGF-β3 + COND were analyzed by qPCR. The cells did not express Nanog , OCT4 , SOX2, or E-cadherin a , the genes responsible for maintaining pluripotency. The differentiated cells expressed chondrogenic genes: type II collagen , SOX5 , SOX6 , SOX9 and NKX3.2 b

Article Snippet: In all analyses, a stable cell line of adult human articular chondrocytes (HC-402-05a) served as a positive control based on the recommendations of the European Collection of Authenticated Cell Cultures (ECACC) for evaluation of the differentiation process in in vitro model systems ( https://www.pheculturecollections.org.uk/products/celllines/primarycells/detail.jsp?refId=06090702&collection=ecacc_nepc ).

Techniques: Derivative Assay

The presence of chondrogenic markers was demonstrated by immunofluorescence analysis. The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND, TGF-β3+ COND did not reveal the presence of pluripotency markers: Nanog, OCT4, E-cadherin ( a ), but they did express desirable chondrogenic proteins: COMP, type II and IX collagen, aggreccan (AGG), SOX6 and SOX9 ( b ). The pluripotency and chondrogenic markers were quantified to better show the differences between particular differentiation protocols ( c and d )

Journal: Stem Cell Reviews

Article Title: Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)

doi: 10.1007/s12015-016-9708-y

Figure Lengend Snippet: The presence of chondrogenic markers was demonstrated by immunofluorescence analysis. The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND, TGF-β3+ COND did not reveal the presence of pluripotency markers: Nanog, OCT4, E-cadherin ( a ), but they did express desirable chondrogenic proteins: COMP, type II and IX collagen, aggreccan (AGG), SOX6 and SOX9 ( b ). The pluripotency and chondrogenic markers were quantified to better show the differences between particular differentiation protocols ( c and d )

Article Snippet: In all analyses, a stable cell line of adult human articular chondrocytes (HC-402-05a) served as a positive control based on the recommendations of the European Collection of Authenticated Cell Cultures (ECACC) for evaluation of the differentiation process in in vitro model systems ( https://www.pheculturecollections.org.uk/products/celllines/primarycells/detail.jsp?refId=06090702&collection=ecacc_nepc ).

Techniques: Immunofluorescence, Derivative Assay