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Cell Applications Inc
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Cell Applications Inc
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China Center for Type Culture Collection
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ScienCell
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European Collection of Authenticated Cell Cultures
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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: Ajuga decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model
doi: 10.3892/etm.2018.5981
Figure Lengend Snippet: Chondrocyte differentiation from hMSCs. hMSCs were cultured for 3 weeks with GM or CD and different concentrations of EADE. Cells were subjected to Alcian blue staining and the absorbance was measured at 630 nm. *P<0.05 and **P<0.01. hMSCs, human mesenchymal stem cells; GM, normal growth medium; CD, chondrogenic differentiation medium; EADE, extra Ajuga decumbens extract.
Article Snippet:
Techniques: Cell Culture, Staining
Journal: Experimental and Therapeutic Medicine
Article Title: Ajuga decumbens stimulates mesenchymal stem cell differentiation and regenerates cartilage in a rabbit osteoarthritis model
doi: 10.3892/etm.2018.5981
Figure Lengend Snippet: Effect of EADE on PGE2 production in chondrocytes. Human chondrocytes were stimulated with IL-1β (1 ng/ml) and treated with 10 or 100 µg/ml EADE. The concentration of PGE2 was significantly increased by IL-1β stimulation, and EADE significantly suppressed this increase. *P<0.05. IL, interleukin; EADE, extra Ajuga decumbens extract; PGE2, prostaglandin E2.
Article Snippet:
Techniques: Concentration Assay
Journal: Stem Cell Reviews
Article Title: Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)
doi: 10.1007/s12015-016-9708-y
Figure Lengend Snippet: HiPSCs were differentiated through four different protocols: a monolayer culture with a set of defined growth factors (GF) (DIRECT protocol); b embryoid bodies (EB) with the exogenous addition of TGF-β3 (TGF-β3 protocol); c EBs in a medium conditioned on the HC-402-05 cell line (COND protocol); and d EBs in a conditioned medium supplemented with TGF-β3 (TGF-β3+ COND protocol). In all cases ( a , b , c , d ), the cells obtained through these differentiation processes presented morphological characteristics of chondrocytes
Article Snippet: In all analyses, a stable cell line of adult
Techniques:
Journal: Stem Cell Reviews
Article Title: Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)
doi: 10.1007/s12015-016-9708-y
Figure Lengend Snippet: The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND and TGF-β3 + COND were analyzed by qPCR. The cells did not express Nanog , OCT4 , SOX2, or E-cadherin a , the genes responsible for maintaining pluripotency. The differentiated cells expressed chondrogenic genes: type II collagen , SOX5 , SOX6 , SOX9 and NKX3.2 b
Article Snippet: In all analyses, a stable cell line of adult
Techniques: Derivative Assay
Journal: Stem Cell Reviews
Article Title: Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs)
doi: 10.1007/s12015-016-9708-y
Figure Lengend Snippet: The presence of chondrogenic markers was demonstrated by immunofluorescence analysis. The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND, TGF-β3+ COND did not reveal the presence of pluripotency markers: Nanog, OCT4, E-cadherin ( a ), but they did express desirable chondrogenic proteins: COMP, type II and IX collagen, aggreccan (AGG), SOX6 and SOX9 ( b ). The pluripotency and chondrogenic markers were quantified to better show the differences between particular differentiation protocols ( c and d )
Article Snippet: In all analyses, a stable cell line of adult
Techniques: Immunofluorescence, Derivative Assay